Non-contact ultrasonic breaker - the best choice for CHIP

Eukaryotic genomic DNA exists in the form of chromatin. Therefore, studying the interaction between protein and DNA in the chromatin environment is the basic way to elucidate the gene expression mechanism of eukaryotes. Chromatin immunoprecipitation assay (CHIP) is currently the only method to study the interaction between DNA and protein in vivo.

The principle of chromatin immunoprecipitation (CHIP):

In the living cell state, the protein-DNA complex is immobilized and randomly cut into small pieces of chromatin within a certain length. Then the complex is precipitated by immunological methods to specifically enrich the DNA of the target protein. Fragments, through the purification and detection of the target fragments, to obtain information on the interaction of proteins with DNA.

Chromatin Co-immunoprecipitation (CHIP) Applications:

Detection of the dynamic effects of trans-factors and DNA in vivo Study of the relationship between various covalent modifications of histones and gene expression The CHIP-on-chip method established by the combination of CHIP and gene chips has been widely used for the high target-specific target cells The combination of flux screening CHIP and the in vivo footprinting method was used to find the in vivo binding site RNA-CHIP of trans factors for studying the role of RNA in gene expression regulation. CHIPseq obtains DNA segment information that interacts with histones, transcription factors, etc. within the entire genome

Diagenode Bioruptor Non-contact Ultrasonic Breaker Safeguards CHIP Sample Processing with the Newest Technology

Broad range of DNA ultrasound to ensure uniform sample handling

Continuous Rotary Sample Processing to Ensure Excellent Repeatability of Chromatin Shearing Results

Rapid ultrasonic crushing speed, shortened boring waiting time, closed sample processing, no infectious droplets, ensuring that the operator's safety sample does not generate bubbles at all, ensuring that the maximum amount of protein sample can be up to 12 and improving the experimental rate

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